Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

Novel avian single-chain fragment variable (scFv) targets dietary gluten and related natural grain prolamins, toxic entities of celiac disease

BACKGROUND Celiac disease (CD) is a chronic, small intestinal inflammatory disease mediated by dietary gluten and related prolamins. The only current therapeutic option is maintenance of a strict life-long gluten-free diet, which implies substantial burden for CD patients. Different treatment regimes might be feasible, including masking of toxic celiac peptides with blocking antibodies or fragm...

Fragment (scFv) Specific for Intercellular Lactobacilli-Expressed Single-Chain Variable

RECOMBINANT SINGLE CHAIN VARIABLE FRAGMENT ANTIBODIES (scFv) AGAINST Pro144-Leu155 FRAGMENT OF HUMAN PROTEIN C.

The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from t...

Human single-chain variable fragment that specifically targets arthritic cartilage

OBJECTIVE To demonstrate that posttranslational modification of type II collagen (CII) by reactive oxygen species (ROS), which are known to be present in inflamed arthritic joints, can give rise to epitopes specific to damaged cartilage in rheumatoid arthritis (RA) and osteoarthritis (OA) and to establish a proof of concept that antibodies specific to ROS-modified CII can be used to target ther...

Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries.

As bioinsecticides Bacillus thuringiensis Cry1C δ-endotoxins also have been used in genetically modified crops worldwide since last century. In this study, single chain variable fragments (scFvs), which could specifically recognize and detect Cry1C in food samples, were isolated from naive phage displayed human antibody libraries (Tomlinson I + J) by iterative affinity selection procedure inste...